wild type glut1 Search Results


glut-1 n (wild-type)  (Promega)
90
Promega glut-1 n (wild-type)
Effect of hypoxia on luciferase reporter constructs after transfection into the murine macrophage-like cell line, RAW 264.7. Cells were exposed to 20.9% (normoxia) or 0.5% O2 (hypoxia) for 16 hours. pGL3, the luciferase reporter construct pGL3 promoter containing the minimal, enhancerless SV40 promoter; OB-HRE1, 22 a luciferase reporter construct controlled by a trimer of the HRE monomer described in Table 1 ▶ (cloned into the multiple cloning site of pGL3 promoter); MMP-7 promoter, 23 a 2.3-kb sequence containing the promoter of the human MMP-7 gene, cloned into the multiple cloning site of pGL2 basic (Promega), a promoterless luciferase reporter construct. <t>GLUT-1-N</t> 21 contains a 184-bp sequence from the rat GLUT-1 promoter cloned into the multiple cloning site of pGL3 promoter (which contains the viral SV40 promoter), and GLUT-1 mol/L is identical apart from a 4-bp mutation (CGTG to ACAT) resulting in ablation of the HRE (HIF binding site). Fold induction values refer to luciferase values obtained under hypoxia divided by those seen under normoxia. Fold inductions (hypoxic luciferase values divided by normoxic values) from four independent transfections are shown. Means ± SEM. *, P < 0.05 compared to pGL3 promoter (Mann Whitney U-test).
Glut 1 N (Wild Type), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+glut1/pmc01868302-160-7-47?v=Promega
Average 90 stars, based on 1 article reviews
glut-1 n (wild-type) - by Bioz Stars, 2026-07
90/100 stars
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reporter plasmids glut1 wild type (wt)  (Shanghai GenePharma)
90
Shanghai GenePharma reporter plasmids glut1 wild type (wt)
Effect of hypoxia on luciferase reporter constructs after transfection into the murine macrophage-like cell line, RAW 264.7. Cells were exposed to 20.9% (normoxia) or 0.5% O2 (hypoxia) for 16 hours. pGL3, the luciferase reporter construct pGL3 promoter containing the minimal, enhancerless SV40 promoter; OB-HRE1, 22 a luciferase reporter construct controlled by a trimer of the HRE monomer described in Table 1 ▶ (cloned into the multiple cloning site of pGL3 promoter); MMP-7 promoter, 23 a 2.3-kb sequence containing the promoter of the human MMP-7 gene, cloned into the multiple cloning site of pGL2 basic (Promega), a promoterless luciferase reporter construct. <t>GLUT-1-N</t> 21 contains a 184-bp sequence from the rat GLUT-1 promoter cloned into the multiple cloning site of pGL3 promoter (which contains the viral SV40 promoter), and GLUT-1 mol/L is identical apart from a 4-bp mutation (CGTG to ACAT) resulting in ablation of the HRE (HIF binding site). Fold induction values refer to luciferase values obtained under hypoxia divided by those seen under normoxia. Fold inductions (hypoxic luciferase values divided by normoxic values) from four independent transfections are shown. Means ± SEM. *, P < 0.05 compared to pGL3 promoter (Mann Whitney U-test).
Reporter Plasmids Glut1 Wild Type (Wt), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+glut1/pm33283896-86-1-13?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
reporter plasmids glut1 wild type (wt) - by Bioz Stars, 2026-07
90/100 stars
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wild type slc2a1  (OriGene)
91
OriGene wild type slc2a1
Effect of hypoxia on luciferase reporter constructs after transfection into the murine macrophage-like cell line, RAW 264.7. Cells were exposed to 20.9% (normoxia) or 0.5% O2 (hypoxia) for 16 hours. pGL3, the luciferase reporter construct pGL3 promoter containing the minimal, enhancerless SV40 promoter; OB-HRE1, 22 a luciferase reporter construct controlled by a trimer of the HRE monomer described in Table 1 ▶ (cloned into the multiple cloning site of pGL3 promoter); MMP-7 promoter, 23 a 2.3-kb sequence containing the promoter of the human MMP-7 gene, cloned into the multiple cloning site of pGL2 basic (Promega), a promoterless luciferase reporter construct. <t>GLUT-1-N</t> 21 contains a 184-bp sequence from the rat GLUT-1 promoter cloned into the multiple cloning site of pGL3 promoter (which contains the viral SV40 promoter), and GLUT-1 mol/L is identical apart from a 4-bp mutation (CGTG to ACAT) resulting in ablation of the HRE (HIF binding site). Fold induction values refer to luciferase values obtained under hypoxia divided by those seen under normoxia. Fold inductions (hypoxic luciferase values divided by normoxic values) from four independent transfections are shown. Means ± SEM. *, P < 0.05 compared to pGL3 promoter (Mann Whitney U-test).
Wild Type Slc2a1, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+glut1/pm25252848-46-27-40?v=OriGene
Average 91 stars, based on 1 article reviews
wild type slc2a1 - by Bioz Stars, 2026-07
91/100 stars
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Image Search Results


Effect of hypoxia on luciferase reporter constructs after transfection into the murine macrophage-like cell line, RAW 264.7. Cells were exposed to 20.9% (normoxia) or 0.5% O2 (hypoxia) for 16 hours. pGL3, the luciferase reporter construct pGL3 promoter containing the minimal, enhancerless SV40 promoter; OB-HRE1, 22 a luciferase reporter construct controlled by a trimer of the HRE monomer described in Table 1 ▶ (cloned into the multiple cloning site of pGL3 promoter); MMP-7 promoter, 23 a 2.3-kb sequence containing the promoter of the human MMP-7 gene, cloned into the multiple cloning site of pGL2 basic (Promega), a promoterless luciferase reporter construct. GLUT-1-N 21 contains a 184-bp sequence from the rat GLUT-1 promoter cloned into the multiple cloning site of pGL3 promoter (which contains the viral SV40 promoter), and GLUT-1 mol/L is identical apart from a 4-bp mutation (CGTG to ACAT) resulting in ablation of the HRE (HIF binding site). Fold induction values refer to luciferase values obtained under hypoxia divided by those seen under normoxia. Fold inductions (hypoxic luciferase values divided by normoxic values) from four independent transfections are shown. Means ± SEM. *, P < 0.05 compared to pGL3 promoter (Mann Whitney U-test).

Journal:

Article Title: Hypoxia-Induced Gene Expression in Human Macrophages

doi:

Figure Lengend Snippet: Effect of hypoxia on luciferase reporter constructs after transfection into the murine macrophage-like cell line, RAW 264.7. Cells were exposed to 20.9% (normoxia) or 0.5% O2 (hypoxia) for 16 hours. pGL3, the luciferase reporter construct pGL3 promoter containing the minimal, enhancerless SV40 promoter; OB-HRE1, 22 a luciferase reporter construct controlled by a trimer of the HRE monomer described in Table 1 ▶ (cloned into the multiple cloning site of pGL3 promoter); MMP-7 promoter, 23 a 2.3-kb sequence containing the promoter of the human MMP-7 gene, cloned into the multiple cloning site of pGL2 basic (Promega), a promoterless luciferase reporter construct. GLUT-1-N 21 contains a 184-bp sequence from the rat GLUT-1 promoter cloned into the multiple cloning site of pGL3 promoter (which contains the viral SV40 promoter), and GLUT-1 mol/L is identical apart from a 4-bp mutation (CGTG to ACAT) resulting in ablation of the HRE (HIF binding site). Fold induction values refer to luciferase values obtained under hypoxia divided by those seen under normoxia. Fold inductions (hypoxic luciferase values divided by normoxic values) from four independent transfections are shown. Means ± SEM. *, P < 0.05 compared to pGL3 promoter (Mann Whitney U-test).

Article Snippet: Two forms of GLUT-1 enhancer were used: GLUT-1 N (wild-type) and GLUT-1 mol/L (bearing a mutated, non-functional HIF-1 binding site), 21 consisting of a 184-bp sequence from the rat GLUT-1 1 promoter cloned into the Kpn I/ Nhe I sites of the pGL3 promoter luciferase reporter construct (Promega, Southampton, UK).

Techniques: Luciferase, Construct, Transfection, Clone Assay, Sequencing, Mutagenesis, Binding Assay, MANN-WHITNEY